Bacterial Growth and Adaptation in Microdroplet Chemostats

Authors


  • This project was co-financed by the EU European Regional Development Fund under the Operational Programme Innovative Economy NanoFun POIG.02.02.00-00-025/09 (to P.G.) and the European Research Council Starting Grant 279647 (to P.G.). Financial support from the Foundation for Polish Science through TEAM (to P.G.) and START programmes (to T.S.K.) is also acknowledged. We also acknowledge support from the Alfred P. Sloan Foundation (fellowship to D.B.W.) and NIH grant 1DP2OD008735-01 (to D.B.W.). We thank Dr. Szymon Kaczanowski for helpful discussions.

Abstract

original image

Getting your bugs in a row: A microfluidic device for manipulating and monitoring the continuous growth of populations of bacteria within microdroplets was developed (see scheme). This device allows for monitoring hundreds of populations of bacteria to study changes in growth rates and the effects of antibiotics, including the evolution of antibiotic resistance in real time.

Ancillary