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Repair of the (6–4) Photoproduct by DNA Photolyase Requires Two Photons

Authors

  • Dr. Junpei Yamamoto,

    Corresponding author
    1. Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531 (Japan)
    • Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531 (Japan)
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  • Ryan Martin,

    1. Département de Chimie, UMR 8640 CNRS-ENS-UPMC, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris (France)
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  • Prof. Shigenori Iwai,

    1. Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531 (Japan)
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  • Dr. Pascal Plaza,

    Corresponding author
    1. Département de Chimie, UMR 8640 CNRS-ENS-UPMC, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris (France)
    • Département de Chimie, UMR 8640 CNRS-ENS-UPMC, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris (France)
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  • Dr. Klaus Brettel

    Corresponding author
    1. UMR 8221, CEA-Institut de Biologie et de Technologie de Saclay, CNRS, Université Paris Sud, 91191 Gif-sur-Yvette (France)
    • UMR 8221, CEA-Institut de Biologie et de Technologie de Saclay, CNRS, Université Paris Sud, 91191 Gif-sur-Yvette (France)
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  • We thank the Japan Society for the Promotion of Science for funding the one-year stay J.Y. made in France. This work was supported by the French Agence Nationale de la Recherche (grant ANR-12-BSV8-0001). We thank Dr. Pavel Müller for helpful discussions.

Abstract

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It takes two (photons) to tango: Single-turnover flash experiments showed that the flavoenzyme (6–4) photolyase uses a successive two-photon mechanism to repair the UV-induced T(6–4)T lesion in DNA (see picture). The intermediate (X) formed by the first photoreaction is likely to be the oxetane-bridged dimer T(ox)T. The enzyme could stabilize the normally short-lived T(ox)T, allowing repair to be completed by the second photoreaction.

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