We thank Dr. Akihiro Ishiwata for his valuable advice as wells as Akemi Takahashi and Satoko Shirahata for their technical assistance. We are also grateful to Dr. Yoshio Sakaguchi and his staff of the Materials Characterization Team in RIKEN Advanced Support Division for elemental analyses.
Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming†
Article first published online: 5 JUN 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 29, pages 7426–7431, July 15, 2013
How to Cite
Koizumi, A., Matsuo, I., Takatani, M., Seko, A., Hachisu, M., Takeda, Y. and Ito, Y. (2013), Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming . Angew. Chem. Int. Ed., 52: 7426–7431. doi: 10.1002/anie.201301613
- Issue published online: 11 JUL 2013
- Article first published online: 5 JUN 2013
- Manuscript Revised: 1 MAY 2013
- Manuscript Received: 25 FEB 2013
- protein folding;
- synthesis design
From the stacks: A novel method for construction of a high-mannose-type glycan library by systematic enzymatic trimming of a single synthetic Man9-based precursor was developed. Efficient chemical synthesis of the tetradecasaccharide common precursor and orthogonal enzymatic trimming to obtain all M8-9 and G1M8-9 derivatives was demonstrated. G=glucose, M=mannose.