Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming

Authors

  • Dr. Akihiko Koizumi,

    1. ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
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  • Prof. Ichiro Matsuo,

    1. Department of Chemistry and Chemical Biology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515 (Japan)
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  • Maki Takatani,

    1. ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
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  • Dr. Akira Seko,

    1. ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
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  • Dr. Masakazu Hachisu,

    1. ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
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  • Dr. Yoichi Takeda,

    Corresponding author
    1. ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
    • ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
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  • Dr. Yukishige Ito

    Corresponding author
    1. ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
    2. Synthetic Cellular Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
    • ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
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  • We thank Dr. Akihiro Ishiwata for his valuable advice as wells as Akemi Takahashi and Satoko Shirahata for their technical assistance. We are also grateful to Dr. Yoshio Sakaguchi and his staff of the Materials Characterization Team in RIKEN Advanced Support Division for elemental analyses.

Abstract

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From the stacks: A novel method for construction of a high-mannose-type glycan library by systematic enzymatic trimming of a single synthetic Man9-based precursor was developed. Efficient chemical synthesis of the tetradecasaccharide common precursor and orthogonal enzymatic trimming to obtain all M8-9 and G1M8-9 derivatives was demonstrated. G=glucose, M=mannose.

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