Exploring the Mechanism of IR–UV Double-Resonance for Quantitative Spectroscopy of Protonated Polypeptides and Proteins


  • We thank EPFL, CH-RU STC program, and SNSF (grant number 130579) for their generous support of this work.


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Spectroscopic fingerprint: Infrared–ultraviolet double resonance photodissociation is used for conformational assignment of the electronic spectra of a cold protonated decapeptide (see picture). A mechanism of the IR–UV depletion spectroscopy is proposed and a procedure of using it for measurements of absolute absorption cross-sections of vibrational transitions is elaborated.