These authors contributed equally to this work.
Unusual Acetylation–Elimination in the Formation of Tetronate Antibiotics†
Article first published online: 18 APR 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 22, pages 5785–5788, May 27, 2013
How to Cite
Kanchanabanca, C., Tao, W., Hong, H., Liu, Y., Hahn, F., Samborskyy, M., Deng, Z., Sun, Y. and Leadlay, P. F. (2013), Unusual Acetylation–Elimination in the Formation of Tetronate Antibiotics . Angew. Chem. Int. Ed., 52: 5785–5788. doi: 10.1002/anie.201301680
This research was supported by grants from the Biotechnology and Biological Sciences Research Council (BBSRC BB/D018943/1), and the 973 and 863 programs from the Ministry of Science and Technology, the National Natural Science Foundation of China, the Ministry of Education. C.K. acknowledges a stipend from the Higher Education Commission of Thailand, W.T. acknowledges funding from the China Postdoctoral Science Foundation (CPSF 2012M521461), and F.H. funding as an EU Marie Curie Postdoctoral Fellow. F.H. is an Emmy Noether Fellow of the Deutsche Forschungsgemeinschaft, and P.F.L. is a Research Awardee of the Alexander von Humboldt Foundation. We thank Prof. Dr. R. D. Süssmuth and Dr. F. Huang for helpful discussions.
- Issue published online: 17 MAY 2013
- Article first published online: 18 APR 2013
- Manuscript Received: 26 FEB 2013
- BBSRC. Grant Number: BB/D018943/1
- Ministry of Science and Technology
- National Natural Science Foundation of China
- Ministry of Education
- China Postdoctoral Science Foundation. Grant Number: CPSF 2012M521461
- Deutsche Forschungsgemeinschaft
- Alexander von Humboldt Foundation
The identity and reactivity of the intermediates in agglomerin biosynthesis were established and the respective roles of the acetyltransferase Agg4 and the eliminating enzyme Agg5 identified (see scheme). It is proposed that enzymes homologous to Agg4 and Agg5 carry out the dehydration steps in all spirotetronate biosynthetic pathways. If this proves correct, it may assist engineering of these pathways.