Duocarmycin Analogues without a DNA-Binding Indole Unit Associate with Aldehyde Dehydrogenase 1A1 and not DNA: A Reply

Authors

  • Prof. Dr. Lutz F. Tietze,

    Corresponding author
    1. Institute of Organic and Biomolecular Chemistry, Georg-August-Universität Göttingen, Tammannstr. 2, 37033 Göttingen (Germany)
    • Lutz F. Tietze, Institute of Organic and Biomolecular Chemistry, Georg-August-Universität Göttingen, Tammannstr. 2, 37033 Göttingen (Germany)

      Stephan A. Sieber, Department Chemie, Center for Integrated Protein Science CIPSM, Institute of Advanced Studies IAS, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)

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  • Prof. Dr. Stephan A. Sieber

    Corresponding author
    1. Department Chemie, Center for Integrated Protein Science CIPSM, Institute of Advanced Studies IAS, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)
    • Lutz F. Tietze, Institute of Organic and Biomolecular Chemistry, Georg-August-Universität Göttingen, Tammannstr. 2, 37033 Göttingen (Germany)

      Stephan A. Sieber, Department Chemie, Center for Integrated Protein Science CIPSM, Institute of Advanced Studies IAS, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)

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Abstract

original image

An open question: Activity-based protein profiling using seco-CBI derivative 1, containing a DNA-binding indole unit, showed that besides binding to DNA, compound 1 also interacts with aldehyde dehydrogenase 1. However, for CBI compounds without an indole unit, the origin of their cytotoxicity has not been identified, yet.

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