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Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA

Authors

  • Naoko Abe,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
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  • Dr. Michio Hiroshima,

    1. Cellular Informatics Laboratory, RIKEN Advanced Science Institute (Japan)
    2. Laboratory for Cell Signaling Dynamics, RIKEN Quantitative Biology Center (Japan)
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  • Hideto Maruyama,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
    2. Faculty of Pharmaceutical Sciences, Hokkaido University (Japan)
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  • Dr. Yuko Nakashima,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
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  • Dr. Yukiko Nakano,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
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  • Prof. Dr. Akira Matsuda,

    1. Faculty of Pharmaceutical Sciences, Hokkaido University (Japan)
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  • Prof. Dr. Yasushi Sako,

    1. Cellular Informatics Laboratory, RIKEN Advanced Science Institute (Japan)
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  • Prof. Dr. Yoshihiro Ito,

    Corresponding author
    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
    • Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)

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  • Prof. Dr. Hiroshi Abe

    Corresponding author
    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
    2. PRESTO (Japan) Science and Technology Agency, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012 (Japan)
    • Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)

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  • H.A. and Y.I. were supported financially by the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT). This work was also supported by a Grant-in-Aid for Scientific Research on Priority Areas, “Lifesurveyor”, from MEXT. We are grateful to the Support Unit for Bio-Material Analysis, RIKEN BSI Research Resources Center, for help with the MS analysis.

Abstract

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Getting the runaround: Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis.

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