Inside Back Cover: Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a Metal-Chelating Unnatural Amino Acid (Angew. Chem. Int. Ed. 18/2013)

Authors

  • Xiaohong Liu,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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    • These authors contributed equally to this work.

  • Jiasong Li,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
    2. School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026 (China)
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    • These authors contributed equally to this work.

  • Cheng Hu,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • Qing Zhou,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • Wei Zhang,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • Meirong Hu,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • Juanzuo Zhou,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • Jiangyun Wang

    Corresponding author
    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
    • Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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Abstract

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A metal-chelating unnatural amino acid (UAA) with an 8-hydroxyquinoline functional group (HqAla) was enzymatically synthesized in one step. In their Communication on page 4805 ff., J. Y. Wang and co-workers show that incorporation of HqAla into fluorescent proteins shifts their emission maxima toward higher wavelengths (red-shifted). This UAA could be applied to engineering metalloproteins and brighter fluorescent proteins, as well as paramagnetic protein NMR spectroscopy.

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