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Photoaffinity Labeling of Small-Molecule-Binding Proteins by DNA-Templated Chemistry

Authors

  • Gang Li,

    1. College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
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  • Ying Liu,

    1. College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
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  • Yu Liu,

    1. College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
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  • Li Chen,

    1. College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
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  • Siyu Wu,

    1. College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
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  • Yang Liu,

    1. College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
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  • Prof. Xiaoyu Li

    Corresponding author
    1. Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, Beijing National Laboratory of Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
    2. College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
    • Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, Beijing National Laboratory of Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Road, Beijing, 100871 (China)
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  • This work was supported by the Ministry of Science and Technology Basic Research Program (grant number 2011CB809100), National Natural Science Foundation of China (grant numbers 21002003, 91013003, and 21272016), the Beijing Nova Program (grant number 2010B002), and the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.

Abstract

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DNA-templated affinity labeling: Accurate characterization of small-molecule (SM)–protein interactions is one of the most important but also very challenging tasks in chemical biology and drug discovery. A novel method named DNA-programmed photoaffinity labeling is reported. The introduction of DNA encoding and template effect enable multiplexed protein labeling by multiple probes.

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