Unidirectional Scaffold-Strand Arrangement in DNA Origami

Authors

  • Dr. Dongran Han,

    1. The Biodesign Institute, Arizona State University, Tempe, AZ 85287 (USA)
    2. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 (USA)
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  • Shuoxing Jiang,

    1. The Biodesign Institute, Arizona State University, Tempe, AZ 85287 (USA)
    2. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 (USA)
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  • Anirban Samanta,

    1. The Biodesign Institute, Arizona State University, Tempe, AZ 85287 (USA)
    2. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 (USA)
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  • Prof. Yan Liu,

    1. The Biodesign Institute, Arizona State University, Tempe, AZ 85287 (USA)
    2. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 (USA)
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  • Prof. Hao Yan

    Corresponding author
    1. The Biodesign Institute, Arizona State University, Tempe, AZ 85287 (USA)
    2. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 (USA)
    • The Biodesign Institute, Arizona State University, Tempe, AZ 85287 (USA)===

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  • This work is supported by a National Science Foundation grant (1104373), an Office of Naval Research grant (N000140911118), an Army Research Office grant (W911NF-11-1-0137) to H.Y. and Y.L., and an Army Research Office MURI award (W911NF-12-1-0420) to H.Y. H.Y. and Y.L. are part of the Center for Bio-Inspired Solar Fuel Production, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC0001016. H.Y. is supported by the Presidential Strategic Initiative Fund from Arizona State University.

Abstract

original image

In a parallel universe: DNA origami structures based on modified parallel double-crossover tiles (see scheme) were constructed and a unidirectional (parallel) arrangement of the scaffold strand (gray lines) was used in the assembly of a variety of 2D and 3D DNA origami structures. This will greatly expand the diversity of DNA origami and enable their assembly into larger structures.

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