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Photoactivatable Chemokines – Controlling Protein Activity by Light

Authors


  • The financial support of the graduate school “Building with Molecules and Nano-objects” (BuildMoNa) and the SFBTRR 67 is kindly acknowledged. We thank Regina Reppich-Sacher and Ramona Öhme for mass spectrometry.

Abstract

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Activation by light: Caged depsipeptides are incorporated as photoresponsive elements into the backbone of the protein SDF-1α by using expressed protein ligation. The disturbed secondary structure is restored after UV treatment by an O→N acyl rearrangement, and the chemotactic potential of the protein is regained. Nvoc=6-nitroveratryloxycarbonyl.

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