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Keywords:

  • dyes/pigments;
  • fluorescence;
  • oxazines;
  • super-resolution microscopy
Thumbnail image of graphical abstract

Brighter dyes in heavy water: A simple and cost-effective method increases the brightness of a whole class of commonly used red-emitting fluorophores, including ATTO655, ATTO680, and ATTO700. Replacing water (H2O) by heavy water (D2O) in the imaging buffer doubles the fluorescence quantum yield of these dyes and significantly improves the localization precision in super-resolution imaging.