This project was supported by the Swiss National Science Foundation through Ambizione fellowship PZ00P3_131935 and by Dormeur Investment Service Ltd. We thank Oliver Hartley for lab space, support, and cell lines, Thomas Huber for helpful comments, and Eric Vauthey for useful discussions and for sharing equipment including the TCSPC detection system.
Improved Super-Resolution Microscopy with Oxazine Fluorophores in Heavy Water†
Article first published online: 4 JUL 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 34, pages 8948–8951, August 19, 2013
How to Cite
Lee, S. F., Vérolet, Q. and Fürstenberg, A. (2013), Improved Super-Resolution Microscopy with Oxazine Fluorophores in Heavy Water. Angew. Chem. Int. Ed., 52: 8948–8951. doi: 10.1002/anie.201302341
- Issue published online: 14 AUG 2013
- Article first published online: 4 JUL 2013
- Manuscript Received: 19 MAR 2013
- National Science Foundation. Grant Number: PZ00P3_131935
- super-resolution microscopy
Brighter dyes in heavy water: A simple and cost-effective method increases the brightness of a whole class of commonly used red-emitting fluorophores, including ATTO655, ATTO680, and ATTO700. Replacing water (H2O) by heavy water (D2O) in the imaging buffer doubles the fluorescence quantum yield of these dyes and significantly improves the localization precision in super-resolution imaging.