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Improved Super-Resolution Microscopy with Oxazine Fluorophores in Heavy Water

Authors

  • Dr. Steven F. Lee,

    Corresponding author
    1. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW (UK)
    • Steven F. Lee, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW (UK)===

      Alexandre Fürstenberg, Department of Human Protein Sciences, University of Geneva, CMU, Rue Michel-Servet 1, 1211 Genève 4 (Switzerland)===

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  • Quentin Vérolet,

    1. Department of Human Protein Sciences, University of Geneva, CMU, Rue Michel-Servet 1, 1211 Genève 4 (Switzerland)
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  • Dr. Alexandre Fürstenberg

    Corresponding author
    1. Department of Human Protein Sciences, University of Geneva, CMU, Rue Michel-Servet 1, 1211 Genève 4 (Switzerland)
    • Steven F. Lee, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW (UK)===

      Alexandre Fürstenberg, Department of Human Protein Sciences, University of Geneva, CMU, Rue Michel-Servet 1, 1211 Genève 4 (Switzerland)===

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  • This project was supported by the Swiss National Science Foundation through Ambizione fellowship PZ00P3_131935 and by Dormeur Investment Service Ltd. We thank Oliver Hartley for lab space, support, and cell lines, Thomas Huber for helpful comments, and Eric Vauthey for useful discussions and for sharing equipment including the TCSPC detection system.

Abstract

original image

Brighter dyes in heavy water: A simple and cost-effective method increases the brightness of a whole class of commonly used red-emitting fluorophores, including ATTO655, ATTO680, and ATTO700. Replacing water (H2O) by heavy water (D2O) in the imaging buffer doubles the fluorescence quantum yield of these dyes and significantly improves the localization precision in super-resolution imaging.

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