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Analysis of Microarrays by MALDI-TOF MS

Authors


  • Funding from the Ministerio de Economía, grants numbers CTQ2008-04444/BQA and CTQ2011-27874, the Basque Government, and the EU, ITN-EuroGlycoArrays grant, is gratefully acknowledged. We thank Juan Etxebarria, CIC biomaGUNE, for help with production and analysis of lectin arrays, Prof. Jim Paulson, Scripps Research Institute, La Jolla and Peter Both, University of Manchester, for providing us with clones for the expression of recombinant glycosyltransferases. MALDI-TOF MS=matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Abstract

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Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium–tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.

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