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Synthesis of Neisseria meningitidis Serogroup W135 Capsular Oligosaccharides for Immunogenicity Comparison and Vaccine Development

Authors

  • Chia-Hung Wang,

    1. Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)
    2. Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 155, Linong Street, Section 2, Taipei, 112 (Taiwan)
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  • Shiou-Ting Li,

    1. Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)
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  • Tzu-Lung Lin,

    1. Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei (Taiwan)
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  • Yang-Yu Cheng,

    1. Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)
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  • Dr. Tsung-Hsien Sun,

    1. Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)
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  • Prof. Jin-Town Wang,

    1. Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei (Taiwan)
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  • Dr. Ting-Jen R. Cheng,

    1. Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)
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  • Prof. Kwok Kong Tony Mong,

    1. Department of Applied Chemistry, National Chiao-Tung University, Hsin-Chu (Taiwan)
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  • Prof. Chi-Huey Wong,

    1. Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)
    2. Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 155, Linong Street, Section 2, Taipei, 112 (Taiwan)
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  • Prof. Chung-Yi Wu

    Corresponding author
    1. Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)
    2. Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 155, Linong Street, Section 2, Taipei, 112 (Taiwan)
    • Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115 (Taiwan)

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  • We thank the CDC of Taiwan for providing the W135 bacteria strain. This work was supported by Academia Sinica and National Science Council, Taiwan. (Grant no NSC 99-2113M-001-008-MY2 and NSC 101-2628M-001-004-MY2 to C.-Y.W.)

Abstract

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Sweetening the deal: N. meningitidis serogroup W135 capsular oligosaccharides were synthesized in lengths from disaccharides to decasaccharides. Sera from mice immunized with these oligosaccharide–protein conjugates were examined by a glycan microarray (see picture) and bactericidal assay for antibody specificity and the ability to kill bacteria.

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