These authors contributed equally to this work.
Rigid DNA Beams for High-Resolution Single-Molecule Mechanics†
Article first published online: 21 JUN 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 30, pages 7766–7771, July 22, 2013
How to Cite
Pfitzner, E., Wachauf, C., Kilchherr, F., Pelz, B., Shih, W. M., Rief, M. and Dietz, H. (2013), Rigid DNA Beams for High-Resolution Single-Molecule Mechanics . Angew. Chem. Int. Ed., 52: 7766–7771. doi: 10.1002/anie.201302727
E.P., C.W., F.K. performed experiments; H.D. and M.R. designed research; E.P., C.W., F.K., H.D., M.R. analyzed data; B.P. built the experimental setup; W.S. provided custom reagents; E.P., C.W., H.D. prepared figures, H.D. wrote the paper; M.R. and W.S. edited the paper. This work was supported by a European Research Council Starting Grant to H.D. (GA no 256270), the Deutsche Forschungsgemeinschaft (DFG) through the programs Collaborative Research Center SFB 863, Center for Integrated Protein Science Munich, and Nanosystems Initiative Munich. F.K. was supported by a stipend from CompInt of Elitenetzwerk Bayern. We thank Florian Praetorius and Johannes Stigler for technical support, and Michael Woodside, Felix Ritort, Ciro Cecconi, Zev Bryant, Thorsten Hugel, and Achillefs Kapanidis for discussions.
- Issue published online: 17 JUL 2013
- Article first published online: 21 JUN 2013
- Manuscript Received: 2 APR 2013
- Funded Access
- European Research Council. Grant Number: 256270
- Deutsche Forschungsgemeinschaft (DFG)
- DNA nanotechnology;
- DNA structures;
- force spectroscopy;
- single-molecule experiments
Bridging the gap: Rigid DNA linkers (blue, see picture) between microspheres (green) for high-resolution single-molecule mechanical experiments were constructed using DNA origami. The resulting DNA helical bundles greatly reduce the noise generated in studies of conformation changes using optical tweezers and were applied to study small DNA secondary structures.