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Rapid Removal of Matrices from Small-Volume Samples by Step-Voltage Nanoelectrospray

Authors

  • Zhenwei Wei,

    1. Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
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  • Shuo Han,

    1. Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
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  • Xiaoyun Gong,

    1. Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
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  • Yaoyao Zhao,

    1. Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
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  • Chengdui Yang,

    1. Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
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  • Prof. Sichun Zhang,

    1. Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
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  • Prof. Xinrong Zhang

    Corresponding author
    1. Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
    • Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)

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  • This research is supported by the 973 program (2013CB933800), the National Natural Science Foundation of China (No. 21027013) and the Ministry of Science and Technology of China (No. 2011YQ090005).

Abstract

original image

Matrix unloaded: By changing from fixed-voltage (left) to step-voltage nanoelectrospray (right), the mass-spectrometric analysis of small-volume physiological samples is possible. Separation and ionization are achieved in one process, which avoids sample loss and dilution and prevents interference by the matrix. The result is high sensitivity even for samples at the nanoliter level.

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