A.R. gratefully acknowledges financial support from the Emmy Noether-Programme of the DFG (RE 2796/2-1) and the Fonds der Chemischen Industrie. J.M.S. thanks the Fonds der Chemischen Industrie for a doctoral fellowship. We acknowledge the MS facility of the Department of Chemistry, University of Hamburg (Dr. Maria Trusch, Christine Christ, and Gaby Graack) for assistance with MALDI-TOF mass spectrometry. We thank Prof. Hahn and his group for providing the DNA template for transcription, Prof. Ralf Ficner (University of Göttingen (Germany)) for the plasmid encoding hTgs1618-853, and Prof. Zhaohui Zhou (Northeastern University, Boston, MA USA) and Prof. Birgit Dräger (University of Halle (Germany)) for plasmids encoding LuxS and MTAN, respectively. The authors declare competing financial interests. Details can be found in the Supporting Information.
A Chemo-Enzymatic Approach for Site-Specific Modification of the RNA Cap†
Article first published online: 21 JUN 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 30, pages 7874–7878, July 22, 2013
How to Cite
Schulz, D., Holstein, J. M. and Rentmeister, A. (2013), A Chemo-Enzymatic Approach for Site-Specific Modification of the RNA Cap . Angew. Chem. Int. Ed., 52: 7874–7878. doi: 10.1002/anie.201302874
- Issue published online: 17 JUL 2013
- Article first published online: 21 JUN 2013
- Manuscript Received: 7 APR 2013
- DFG. Grant Number: RE 2796/2-1
- Fonds der Chemischen Industrie
- AdoMet analogues;
- click chemistry;
Capped and gowned: A two-step approach can be used to site-specifically modify the 5′-cap of eukaryotic mRNAs. First, a trimethylguanosinesynthase variant recognizes the m7G cap structure and introduces bioorthogonal groups using S-adenosyl-L-methionine-based cosubstrates. Then, the enzymatically introduced reporter groups are further modified by thiol–ene or CuAAC click chemistry (see scheme).