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Keywords:

  • AdoMet analogues;
  • click chemistry;
  • mRNA;
  • trimethylguanosinesynthase
Thumbnail image of graphical abstract

Capped and gowned: A two-step approach can be used to site-specifically modify the 5′-cap of eukaryotic mRNAs. First, a trimethylguanosinesynthase variant recognizes the m7G cap structure and introduces bioorthogonal groups using S-adenosyl-L-methionine-based cosubstrates. Then, the enzymatically introduced reporter groups are further modified by thiol–ene or CuAAC click chemistry (see scheme).