Environment-Sensitive Fluorescent Turn-On Probes Targeting Hydrophobic Ligand-Binding Domains for Selective Protein Detection

Authors

  • Yu-De Zhuang,

    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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    • These authors contributed equally to this work.

  • Po-Yi Chiang,

    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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    • These authors contributed equally to this work.

  • Chia-Wen Wang,

    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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  • Prof. Dr. Kui-Thong Tan

    Corresponding author
    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
    2. Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
    • Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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  • We are grateful to the National Science Council (Grant No.: 100-2113-M007-018-MY2) and Ministry of Education (Grant No.: 101N2011E1), Taiwan (ROC) for financial support.

Abstract

original image

Bind and shine: An approach for the selective detection of both enzymes and non-enzymatic proteins using an environment-sensitive fluorescent turn-on probe is described (see scheme). This approach targets the hydrophobic ligand-binding domain of the target protein to trigger the fluorescence turn-on and was shown to be specific for the targeted protein.

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