Cover Picture: A Facile Strategy for Selective Incorporation of Phosphoserine into Histones (Angew. Chem. Int. Ed. 22/2013)

Authors

  • Sangsik Lee,

    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    Search for more papers by this author
  • Seunghee Oh,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    Search for more papers by this author
  • Aerin Yang,

    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    Search for more papers by this author
  • Jihyo Kim,

    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    Search for more papers by this author
  • Prof. Dieter Söll,

    1. Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Ave, New Haven, CT 06511 (USA)
    Search for more papers by this author
  • Prof. Daeyoup Lee,

    Corresponding author
    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    • Daeyoup Lee, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

      Hee-Sung Park, Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

    Search for more papers by this author
  • Prof. Hee-Sung Park

    Corresponding author
    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    • Daeyoup Lee, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

      Hee-Sung Park, Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

    Search for more papers by this author

Abstract

original image

Selective phosphoserine incorporation is described by H.-S. Park et al. in their Communication on page 5771 ff. A general strategy for producing recombinant histones with site-specific serine phosphorylation has been developed by engineering phosphoseryl-tRNA synthethase (SepRS) and elongation factor Tu (EF-Tu). This method should facilitate the study of histone phosphorylation and cross-regulatory mechanisms.

Cartoon 1.

Selective phosphoserine incorporation is described by H.-S. Park et al. in their Communication on page 5771 ff. A general strategy for producing recombinant histones with site-specific serine phosphorylation has been developed by engineering phosphoseryl-tRNA synthethase (SepRS) and elongation factor Tu (EF-Tu). This method should facilitate the study of histone phosphorylation and cross-regulatory mechanisms.

Natural Products

In their Communication on page 5726 ff., J. Tulla-Puche, F. Albericio, et al. describe the solid-phase synthesis of the complex cyclothiodepsipeptide thiocoraline. The applied phenylacetamidomethyl protecting group was cleaved by an immobilized enzyme.

Thumbnail image of

Synthetic Methods

In their Communication on page 5795 ff., Y. Huang et al. describe a general procedure for the synthesis of unprotected indoles. By using a cleavable triazene as the directing group, a C[BOND]H annulation with excellent regioselectivity was accomplished.

Thumbnail image of

Cell Internalization

The internalization of proteins and nanoparticles in living cells can be quantified by using a new DNA nanosensor, as described by A. P. R. Johnston and H. Liu on page 5744 ff. This technique enables multicolor assays and studies in primary cells without compromising sensitivity or quantification.

Thumbnail image of

Ancillary