Inside Back Cover: High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase (Angew. Chem. Int. Ed. 21/2013)

Authors

  • Dr. Ryan Lauchli,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)
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  • Dr. Kersten S. Rabe,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)
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  • Karolina Z. Kalbarczyk,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)
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  • Amulya Tata,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)
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  • Dr. Thomas Heel,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)
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  • Rebekah Z. Kitto,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)
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  • Prof. Dr. Frances H. Arnold

    Corresponding author
    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)
    • Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125 (USA)

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Abstract

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Active terpene synthase mutants are generally identified by using lower-throughput GC-based methods. In their Communication on page 5571 ff., F. H. Arnold and co-workers describe a terpene synthase substrate that enables cyclization activity to be screened in a high-throughput manner. The screening protocol allows the identification of active mutants by the brilliant purple color that these mutants generate in the assay.

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