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Light-Regulated Stapled Peptides to Inhibit Protein–Protein Interactions Involved in Clathrin-Mediated Endocytosis

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  • We are grateful to A. Adeva for help with peptide synthesis at the initial stages of the project, to A. Lladó and L. Bardia for technical support with cell imaging and useful discussions, and to CCiTUB Flow Cytometry Facility for technical support. We thank S. J. Royle for providing the LCa–mRFP plasmid, H. McMahon for the β-appendage plasmid, T. Kirchhausen for the BSC-1 cell line. We acknowledge financial support from the Human Frontier Science Program through a Career Development Award (CDA022/2006), from the European Research Council through the “Opticalbullet” Starting Grant (ERC-StG-210355/2007), from the European Commission through the “Focus” ICT-FET grant (FP7-ICT-2009-270483), from the Ministry of Education through grant CTQ2008-06160, and a FPU fellowship (to A.M.-Q.), and from the RecerCaixa and Marató de TV3 foundations.

Abstract

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Control of membrane traffic: Photoswitchable inhibitors of protein–protein interactions were applied to photoregulate clathrin-mediated endocytosis (CME) in living cells. Traffic light (TL) peptides acting as “stop” and “go” signals for membrane traffic can be used to dissect the role of CME in receptor internalization and in cell growth, division, and differentiation.

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