Positron Emission Tomography Imaging of Drug-Induced Tumor Apoptosis with a Caspase-Triggered Nanoaggregation Probe

Authors

  • Dr. Bin Shen,

    1. Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. Jongho Jeon,

    1. Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. Mikael Palner,

    1. Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)
    Search for more papers by this author
  • Dr. Deju Ye,

    1. Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)
    Search for more papers by this author
  • Dr. Adam Shuhendler,

    1. Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)
    Search for more papers by this author
  • Prof. Frederick T. Chin,

    Corresponding author
    1. Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)
    • Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)

    Search for more papers by this author
  • Prof. Jianghong Rao

    Corresponding author
    1. Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)
    • Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305-5484 (USA)

    Search for more papers by this author

  • This work has been supported by the Stanford University National Cancer Institute (NCI) Centers of Cancer Nanotechnology Excellence (grant number 1U54CA151459-01), the NCI ICMIC@Stanford (grant number 1P50CA114747-06), and an IDEA award from the Department of Defense Breast Cancer Research Program (grant number W81XWH-09-1-0057). M.P. is grateful to the postdoctoral fellowship support from the Danish Cancer Foundation, and A.S. is supported by a postdoctoral fellowship from the Susan Komen Breast Cancer Foundation. The use of Stanford Small Animal Imaging Facility is acknowledged.

Abstract

original image

Drug Design: An 18F-labeled caspase-3-sensitive nanoaggregation positron emission tomography tracer was prepared and evaluated for imaging the caspase-3 activity in doxorubicin-treated tumor xenografts. Enhanced retention of the 18F activity in apoptotic tumors is achieved through intramolecular macrocyclization and in situ aggregation upon caspase-3 activation (see picture).

Ancillary