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N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics

Authors

  • Yanbo Pan,

    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (China)
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  • Prof. Dr. Mingliang Ye,

    Corresponding author
    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
    • CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)

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  • Dr. Liang Zhao,

    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
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  • Kai Cheng,

    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (China)
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  • Mingming Dong,

    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (China)
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  • Chunxia Song,

    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (China)
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  • Hongqiang Qin,

    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (China)
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  • Dr. Fangjun Wang,

    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
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  • Prof. Dr. Hanfa Zou

    Corresponding author
    1. CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)
    • CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 (China)

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  • This work is funded in part by the Creative Research Group Project of NSFC (21021004), NSFC (21235006, 21275142), the China State Key Basic Research Program Grant (2013CB911202, 2012CB910101, 2012CB910604), and National Key Special Program on Infection diseases (2012ZX10002009-011).

Abstract

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Two times an enzyme: Trypsin was first used as a protease to catalyze the digestion of proteins and then as a ligase to catalyze the linking of isotopically labeled amino acids to the N termini of tryptic peptides for quantitative proteomics. Reliable and accurate quantification of proteins was demonstrated for model proteins as well as proteome samples.

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