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Keywords:

  • DNA origami;
  • DNA structures;
  • formamide;
  • room temperature assembly;
  • single-stranded tiles
Thumbnail image of graphical abstract

Efficient self-assembly: Self-assembly of DNA nanostructures at room temperature was achieved by incubating the component strands in the presence of a denaturing agent (30–40% formamide). This isothermal method is efficient for assembly of both DNA origami (purple, see scheme) and single-stranded tile (SST, blue) structures, as well as an SST ribbon growing on an origami template.