Self-Assembly of DNA Origami and Single-Stranded Tile Structures at Room Temperature

Authors

  • Dr. Zhao Zhang,

    1. Center for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C (Denmark)
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    • These authors contributed equally to this work.

  • Jie Song,

    1. Center for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C (Denmark)
    2. School of Physical Science and Technology, Lanzhou University, Lanzhou 730000 (P. R. China)
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    • These authors contributed equally to this work.

  • Prof. Flemming Besenbacher,

    1. Center for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C (Denmark)
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  • Dr. Mingdong Dong,

    Corresponding author
    1. Center for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C (Denmark)
    • Center for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C (Denmark)

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  • Prof. Kurt V. Gothelf

    Corresponding author
    1. Center for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C (Denmark)
    • Center for DNA Nanotechnology (CDNA) at the Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C (Denmark)

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  • We thank William Shih and Yonggang Ke for providing the DNA strands of 24HB, as well as Niels Voigt and Christian Rosen for communication. This research was supported by grants from the Danish National Research Foundation to the Center for DNA Nanotechnology and the Danish Council for Independent Research for an Eliteforsk Award. M.D. acknowledges financial support from the Carlsberg Foundation, and the Villum Foundation.

Abstract

original image

Efficient self-assembly: Self-assembly of DNA nanostructures at room temperature was achieved by incubating the component strands in the presence of a denaturing agent (30–40% formamide). This isothermal method is efficient for assembly of both DNA origami (purple, see scheme) and single-stranded tile (SST, blue) structures, as well as an SST ribbon growing on an origami template.

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