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Micropatterned Photodegradable Hydrogels for the Sorting of Microbeads and Cells

Authors

  • Christian Siltanen,

    1. Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA)
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  • Dr. Dong-Sik Shin,

    Corresponding author
    1. Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA)
    • Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA)

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  • Prof. Julie Sutcliffe,

    1. Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA)
    2. Division of Hematology/Oncology, Department of Internal Medicine, Center for Molecular and Genomic Imaging, University of California Davis, Davis, CA 95616 (USA)
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  • Prof. Alexander Revzin

    Corresponding author
    1. Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA)
    • Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA)

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  • The authors gratefully acknowledge Sven Hausner and Kevin Lau for their assistance with experimental design. This work was supported by an NIH grant (C4EB012836-01) to J.S. and an NSF EFRI grant (0937997) to A.R.

Abstract

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Sorted out: Soft-lithography micropatterning of a photodegradable poly(ethylene glycol) hydrogel was utilized to sort cell-bound microbeads. Biofunctionalized microbeads were patterned, screened for cell binding activity, and isolated from arrays by UV irradiation. The technique is a simple approach for sorting and downstream processing of cultured cells.

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