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ESI Hydrogen/Deuterium Exchange Can Count Chemical Forms of Heteroatom-Bound Hydrogen

Authors

  • Sebastian Tittebrandt,

    1. Molecular Structure Analysis, German Cancer Research Center, 69120 Heidelberg (Germany)
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  • Dr. Marina Edelson-Averbukh,

    1. Molecular Structure Analysis, German Cancer Research Center, 69120 Heidelberg (Germany)
    2. Current address: Department of Chemistry, Imperial College, London SW7 2AZ (UK)
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  • Prof. Dr. Bernhard Spengler,

    1. Institute for Inorganic and Analytical Chemistry, University Giessen, 35392 Giessen (Germany)
    2. Current address: Department of Chemistry, Imperial College, London SW7 2AZ (UK)
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  • Prof. Dr. Wolf D. Lehmann

    Corresponding author
    1. Molecular Structure Analysis, German Cancer Research Center, 69120 Heidelberg (Germany)
    • Molecular Structure Analysis, German Cancer Research Center, 69120 Heidelberg (Germany)===

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  • Financial support by the State of Hesse (LOEWE Research Focus “AmbiProbe”) is gratefully acknowledged.

Abstract

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Out for the count: Hydrogen–deuterium exchange (HDX) performed in ESI droplets can distinguish chemically distinct forms of labile hydrogen. NanoESI exchanged mainly O-bound hydrogen atoms, whereas ESI also exchanged a subset of N-bound hydrogen atoms. The exchange behavior could be predicted. Thus, the combined use of nanoESI- and ESI-HDX can be applied to count chemically different forms of labile hydrogen atoms.

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