Out for the count: Hydrogen–deuterium exchange (HDX) performed in ESI droplets can distinguish chemically distinct forms of labile hydrogen. NanoESI exchanged mainly O-bound hydrogen atoms, whereas ESI also exchanged a subset of N-bound hydrogen atoms. The exchange behavior could be predicted. Thus, the combined use of nanoESI- and ESI-HDX can be applied to count chemically different forms of labile hydrogen atoms.
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