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Keywords:

  • C[BOND]H activation;
  • cytochromes;
  • enzyme catalysis;
  • protein engineering;
  • synthetic biology
Thumbnail image of graphical abstract

Nitrogen activation: Though P450 enzymes are masters of oxygen activation and insertion into C[BOND]H bonds, their ability to use nitrogen for the same purpose has so far not been explored. Engineered variants of cytochrome P450BM3 have now been found to catalyze intramolecular C[BOND]H aminations in azide substrates. Mutations to two highly conserved residues significantly increased this activity.