We acknowledge Silke Wiesner for advice and suggestions to the manuscript. We thank Ancilla Neu, Lisa Strittmatter, and other group members for discussions. This study was supported by the Max Planck Society and a Marie Curie Reintegration Grant (FP7/2007-2013, grant agreement no. 239164) to R.S.
LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes†
Article first published online: 14 AUG 2013
© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of Creative Commons the Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Angewandte Chemie International Edition
Volume 52, Issue 43, pages 11401–11405, October 18, 2013
How to Cite
Mund, M., Overbeck, J. H., Ullmann, J. and Sprangers, R. (2013), LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes. Angew. Chem. Int. Ed., 52: 11401–11405. doi: 10.1002/anie.201304914
- Issue published online: 14 OCT 2013
- Article first published online: 14 AUG 2013
- Manuscript Received: 7 JUN 2013
- Funded Access
- Max Planck Society
- Marie Curie Reintegration Grant. Grant Number: 239164
- isotopic labeling;
- LSm complex;
- methyl TROSY;
- NMR spectroscopy;
- protein structures
Seeing the big picture: Asymmetric macromolecular complexes that are NMR active in only a subset of their subunits can be prepared, thus decreasing NMR spectral complexity. For the hetero heptameric LSm1–7 and LSm2–8 rings NMR spectra of the individual subunits of the complete complex are obtained, showing a conserved RNA binding site. This LEGO-NMR technique makes large asymmetric complexes accessible to detailed NMR spectroscopic studies.