This research was supported by grants from Baxter Healthcare and NIH (grant number R37 DE014193). T.S.S. was funded by a National Science Foundation graduate fellowship (grant number GRFP 2011124091) and the Ryan Fellowship. We would like to thank Prof. Vinzenz Unger for technical advice and for use of the anaerobic glove box.
Colorless Multifunctional Coatings Inspired by Polyphenols Found in Tea, Chocolate, and Wine†
Article first published online: 22 AUG 2013
© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of Creative Commons the Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Angewandte Chemie International Edition
Volume 52, Issue 41, pages 10766–10770, October 4, 2013
How to Cite
Sileika, T. S., Barrett, D. G., Zhang, R., Lau, K. H. A. and Messersmith, P. B. (2013), Colorless Multifunctional Coatings Inspired by Polyphenols Found in Tea, Chocolate, and Wine . Angew. Chem. Int. Ed., 52: 10766–10770. doi: 10.1002/anie.201304922
- Issue published online: 1 OCT 2013
- Article first published online: 22 AUG 2013
- Manuscript Revised: 25 JUL 2013
- Manuscript Received: 7 JUN 2013
- Funded Access
- Baxter Healthcare
- NIH. Grant Number: R37 DE014193
- National Science Foundation. Grant Number: GRFP 2011124091
- Ryan Fellowship
- multifunctional materials;
- surface chemistry
Phenols and polyphenols are widely distributed in plant tissues, where they are linked to diverse biological functions such as chemical defense, pigmentation, structural support, and prevention of radiation damage.1 Members of this large family of compounds include epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC), and tannic acid (TA), to name a few (Figure 1 B). Plant polyphenols display a rich and complex spectrum of physical and chemical properties,1a giving rise to broad chemical versatility including absorption of UV radiation, radical scavenging, and metal ion complexation. In addition, significant attention has been given to the purported health benefits associated with consumption of foods and beverages rich in plant polyphenols.2
Of interest to us in this report is the strong solid–liquid interfacial activity exhibited by plant polyphenols, a property that is reflected in their historical use as binding agents (lignin) and in leather manufacturing (vegetable tannins). The high dihydroxyphenyl (catechol) and trihydroxyphenyl (gallic acid, GA) content of plant polyphenols further attracted our interest in the context of surface modification, as catechols are known to strongly bind to surfaces through covalent and noncovalent interactions3 and are prominent constituents of marine polyphenolic protein adhesives.4 Inspired by the high catecholamine content of mussel adhesive proteins and by the involvement of catecholamines in melanin biosynthesis, in situ oxidative polymerization of dopamine at alkaline pH was recently discovered as a universal route for deposition of multifunctional coatings onto surfaces.5 Although polydopamine (pDA) is simple to apply to substrates, deposits on a wide range of materials and offers many potential applied uses, the high costs of dopamine and the characteristically dark color of pDA coatings may be impediments for some practical applications. While a colorless pDA approach was recently reported,6 the method employed a 2:1 mixture of 2-bromoisobutyryl-substituted dopamine to dopamine and was achieved at the expense of a roughly fourfold reduction in coating thickness.
Herein, we describe the use of low-cost plant polyphenols, their building blocks and trihydroxyphenyl-containing molecules as precursors for the formation of multifunctional coatings. In contrast to previous studies where plant polyphenols have been investigated as monolayer adsorbates or as ingredients in multicomponent coatings,7 our strategy features either a plant polyphenol (TA) or a simple phenolic mimic (pyrogallol, PG) as the sole coating precursor. Plant polyphenol-inspired coatings retain many of the advantages of pDA and deposit under similar conditions, yet are colorless and derived in some cases from reagents hundredfold less costly than dopamine.
The coating potential of plant polyphenols was first illustrated with a simple experiment involving unadulterated tea and wine, yielding the surprising finding that thin polyphenol coatings form spontaneously on surfaces exposed to these polyphenol-rich beverages (Figure 1). A freshly prepared green tea infusion left undisturbed in a covered porcelain cup for several hours and then rinsed with tap water, appeared to leave no residue or produce any observable color changes to the cup. However, the presence of a thin polyphenol coating was revealed by immersing the tea cup in a AgNO3 solution, resulting in the deposition of a dark metallic silver film on the surface, presumably through a redox reaction between Ag+ ions and the polyphenolic coating (Figure 1 C). The tea bag used to prepare the infusion also appeared unchanged but darkened markedly upon treatment with AgNO3. The presence of surface-bound silver nanoparticles was evident upon inspection using scanning electron microscopy (SEM, Figure S2). Similarly, red wine also deposited an indiscernible polyphenol coating on glass that was visible only after treatment with AgNO3 (Figure 1 D).
Next we investigated crude extracts of red wine (RWE), cacao bean (CBE), and green tea (GTE) for their ability to form polyphenol films on polymeric, metallic, and native-oxide surfaces. Aqueous extracts were prepared and assayed for gallic acid equivalents (GAE),8 revealing the high polyphenol content of the crude extracts (Figure S3). Immersion of polysulfone (PS) substrates into dilute solutions of RWE, GTE, and CBE (2 mg mL−1) left a uniform colorless polyphenol coating on the surface, attenuating the underlying substrate signal as measured by XPS (Figure 2 A). Coating deposition was most effective from buffered saline (0.6 M NaCl, pH 7.8) as compared to pure water, with coatings derived from CBE totally obscuring the S2p signal of the substrate, indicating thickness of about 10 nm or more (Figure S4).
We subsequently investigated pure ECG and EGCG, two flavan-3-ols known to be present in high concentration in green tea, by immersing polycarbonate (PC, Figure 2 B) and titanium dioxide (TiO2, Figure S5) substrates in buffered saline containing 0.1–0.5 mg mL−1 of each compound. Chemical analysis of the resulting surfaces by XPS (Figure S5) and by silver visualization as described above showed that EGCG and ECG formed colorless coatings in a similar fashion (Figure 2 B), suggesting that the coatings derived from crude GTE may be a result of the presence of EGCG, ECG or related polyphenols.
Taking the results of these exploratory experiments into consideration, as well as the fact that the trihydroxyphenyl functional group is a common feature of EGCG, ECG, and other plant polyphenols, we selected PG and the gallate ester-rich TA as simple, readily available and low-cost precursors for deposition of multifunctional coatings on substrates. Thin coatings spontaneously deposited onto gold and titanium dioxide from PG and TA solutions, increasing in thickness with incubation time (Figure 2 C) and precursor concentration (Figure S6). Maximal thickness of approximately 20 nm occurred after 8 h of incubation in PG, and 65 nm in TA (2 mg mL−1 in buffered saline). Furthermore, the versatility of the coating method was illustrated by forming coatings on a variety of organic and inorganic substrates, including metals such as stainless steel (SS) and hydrophobic polymers like polytetrafluoroethylene (PTFE; Figure 2 D and Figure S7). Coatings derived from PG appeared to induce only minor changes in surface roughness relative to the bare substrate, as assessed by SEM (Figure S8), while being sufficiently thick as to completely mask the XPS signal of most underlying substrates (Figure 2 D).
TA-modified surfaces especially, and to some extent coatings derived from PG, appear colorless when compared to pDA coatings of comparable thickness (Figure 2 E and Figure S9). This was confirmed with UV/Vis measurements, which showed little change in the absorption spectrum of TA-coated PC (Figure S10). Exposure of coatings derived from TA and PG to aqueous AgNO3 (100 mM) afforded electroless silver metallization as indicated by the presence of silver nanoparticles bound to the surface of the coating (Figures S11–S13).
Oxidation of plant polyphenols is known to lead to the formation of higher molecular weight species9 and these reactions are intimately involved in the browning of fruits and vegetables, in leather tanning, and in the fermentation of tea leaves.10 We suspect that oxidation reactions are responsible for the formation of coatings from ECGC, ECG, PG, and TA. Similar to coatings derived from dopamine polymerization,5 plant polyphenol-inspired coatings form spontaneously at mildly alkaline pH (7.8) in the presence of available dissolved oxygen (Figure S14), likely through phenolate ion intermediaries. Although further studies will be necessary to understand the exact mechanism of coating formation, we surmise that oxidation followed by oligomerization decreases the solubility of plant polyphenols and combined with their inherent affinity toward surfaces, ultimately leads to surface deposition. Mass spectrometry analysis of PG coatings and polymerization solutions indicated the presence of high-molecular-weight species, consistent with this general model (Figure S15).
Motivated by the many diverse functions of plant polyphenols in nature, we found that polyphenol coatings could display numerous useful functional properties. For example, emulating the natural role of plant polyphenols in biological defense,11 we found that polyphenol coatings derived from TA (Figure 3 A) and PG (Figure S16) displayed strong contact-based antibacterial properties against both gram-negative and gram-positive strains. P. aeruginosa and S. aureus viability was reduced at least 30-fold upon exposure to PC modified with TA for 3 h (Figure 3 A). Importantly, the same coatings were nontoxic to mammalian cells, as exposure of NIH 3T3 fibroblast cells to TA- and PG-coated substrates revealed no observable cytotoxicity.
Resistance to fouling by bacterial and mammalian cells was conferred by grafting antifouling polymers onto polyphenol coatings, exploiting the proclivity of polyphenols to covalently react with nucleophilic groups of polypeptides and other molecules.1a, 12 Grafting of monofunctional thiol- or amine-terminated poly(ethylene glycol) (mPEG-SH and mPEG-NH2) was performed under the same deposition conditions that were used to deposit PG coatings (Figure S17), and the resultant surfaces resisted attachment of NIH 3T3 fibroblasts (Figure 3 B and Figure S18), P. aeruginosa, and S. aureus over a 24 h period (Figure 3 C).
Additionally, our coatings display one of the most celebrated features of plant polyphenols: the ability to scavenge radical and non-radical reactive oxygen species (ROS).13 We examined the antioxidant properties of polyphenol-coated fumed silica (FS) nanoparticles and flat tissue culture polystyrene (TCPS) surfaces, using both chemical and cell-based assays. Radical scavenging properties were assessed using a stable radical assay based on 2,2-diphenyl-1-picrylhydrazyl (DPPH; Figure S19), and antioxidant behavior was probed through β-carotene degradation (Figure 3 D). A significant antioxidant effect was observed for polyphenol coatings derived from TA, although PG coatings were less effective (Figure 3 D). Intracellular ROS production in NIH 3T3 fibroblasts was notably attenuated in the presence of unmodified and TA-modified fumed silica nanoparticles (Figure S20). Most interestingly, measured intracellular ROS levels for 3T3 cells grown on TA-coated TCPS were substantially lower than for cells grown on bare TCPS (Figure 3 E). The reduction in ROS levels cannot be attributed to dissolved polyphenols, as culture media exposed to TA-coated TCPS failed to display measureable GAE content. This result implies an intracellular protective effect for cells cultivated on an extracellular polyphenol film, a finding that may have important implications for modulating the acute inflammatory response to implanted medical devices. We speculate that membrane-associated esterases could hydrolytically degrade portions of the coating, which then become internalized within the cell and provide protective effects similar to those observed by soluble polyphenols.
Finally, we demonstrated the use of plant polyphenol-inspired coatings for modulation of the optical properties of inorganic nanoparticles by depositing a PG coating onto cetyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (Au NRs) displaying a native longitudinal surface plasmon resonance (SPR) at 895 nm. Suspending CTAB-stabilized Au NRs in 0.1 mg mL−1 PG resulted in displacement of the CTAB ligand and formation of a thin adherent PG coating on the Au NRs (Au-PG NRs). Secondary electron imaging revealed a less electron-dense 5 nm thick PG adlayer visible against the metallic core (Figure S21). Subsequent addition of AgNO3 to the suspension yielded bimetallic nanorods (Au-PG-Ag), with energy-dispersive X-ray spectroscopy (EDS) revealing the constructs to consist of a gold core and a silver shell (Figure 4 A). The thickness of the silver shell increased with concentration of AgNO3, facilitating fine-tuning of the longitudinal SPR wavelength through a blue shift of the SPR (Figure 4 B and C). The ability to tune optical properties of nanoparticles is desirable in a variety of contexts, including medical applications of metallic nanoparticles where the SPR is often employed for diagnostic and therapeutic purposes.14
In summary, we described a novel bioinspired approach to the formation of colorless multifunctional coatings, exploiting the versatility and multifunctionality of plant polyphenols and their mimics. We believe that the simplicity and versatility of the strategy, combined with the low costs and wide availability of TA and PG, are attractive features that will lead to many additional applications beyond the ones we have described. Plant polyphenol-inspired coatings are not only possible through the use of low-cost precursors like PG and TA, but also through the direct use of extracts from polyphenol-rich foods, therefore representing a “green” family of surface modification strategies.
- 1aPractical polyphenolics: from structure to molecular recognition and physiological action, Cambridge University Press, 1998;,
- 2bTea in health and disease prevention, Elsevier, London, 2013;,
- 12aPlant polyphenols: vegetable tannins revisited, Cambridge University Press, Cambridge, 1989;,
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