Coupling Biocatalysis with Molecular Imprinting in a Biomimetic Sensor

Authors

  • Dr. Aysu Yarman,

    1. Fraunhofer Institute for Biomedical Engineering, Am Mühlenberg 13, 14476 Potsdam (Germany)
    2. Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24–25, 14476 Potsdam (Germany)
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  • Prof. Dr. Frieder W. Scheller

    Corresponding author
    1. Fraunhofer Institute for Biomedical Engineering, Am Mühlenberg 13, 14476 Potsdam (Germany)
    2. Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24–25, 14476 Potsdam (Germany)
    • Fraunhofer Institute for Biomedical Engineering, Am Mühlenberg 13, 14476 Potsdam (Germany)

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  • We thank Alexander Christmann for the AFM investigations and the BMBF (0311993) of Germany for financial support. This research forms part of UniCat, a Cluster of Excellence in the field of catalysis that is coordinated by the Technical University of Berlin and supported financially by the Deutsche Forschungsgemeinschaft (DFG) within the framework of the German Excellence Initiative (EXC 314).

Abstract

original image

Make it simple: A molecularly imprinted electropolymer was combined with an enzyme in a catalytic biomimetic sensor that enabled interference-free detection of the drug aminopyrine (AP) at submicromolar concentrations in the presence of ascorbic acid and uric acid within 15 s. The sensor functioned by the peroxide-dependent conversion of AP in a layer above a product-imprinted electropolymer on an indicator electrode (see picture).

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