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Neural Stem Cells Harvested from Live Brains by Antibody-Conjugated Magnetic Nanoparticles

Authors

  • Dr. C. N. P. Lui,

    1. Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)
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  • Y. P. Tsui,

    1. Department of Biochemistry and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)
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  • A. S. L. Ho,

    1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)
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  • Prof. D. K. Y. Shum,

    Corresponding author
    1. Department of Biochemistry and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)
    • D. K. Y. Shum, Department of Biochemistry and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      Y. S. Chan, Department of Physiology and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      H. W. Li, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

      S. C. Edman Tsang, Department of Chemistry, University of Oxford, Oxford, OX1 3QR (UK)

      K. K. L. Yung, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

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  • Prof. Y. S. Chan,

    Corresponding author
    1. Department of Physiology and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)
    • D. K. Y. Shum, Department of Biochemistry and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      Y. S. Chan, Department of Physiology and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      H. W. Li, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

      S. C. Edman Tsang, Department of Chemistry, University of Oxford, Oxford, OX1 3QR (UK)

      K. K. L. Yung, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

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  • C. T. Wu,

    1. Department of Chemistry, University of Oxford, Oxford, OX1 3QR (UK)
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  • Dr. H. W. Li,

    Corresponding author
    1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)
    • D. K. Y. Shum, Department of Biochemistry and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      Y. S. Chan, Department of Physiology and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      H. W. Li, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

      S. C. Edman Tsang, Department of Chemistry, University of Oxford, Oxford, OX1 3QR (UK)

      K. K. L. Yung, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

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  • Prof. S. C. Edman Tsang,

    Corresponding author
    1. Department of Chemistry, University of Oxford, Oxford, OX1 3QR (UK)
    • D. K. Y. Shum, Department of Biochemistry and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      Y. S. Chan, Department of Physiology and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      H. W. Li, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

      S. C. Edman Tsang, Department of Chemistry, University of Oxford, Oxford, OX1 3QR (UK)

      K. K. L. Yung, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

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  • Prof. K. K. L. Yung

    Corresponding author
    1. Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)
    • D. K. Y. Shum, Department of Biochemistry and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      Y. S. Chan, Department of Physiology and Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (China)

      H. W. Li, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

      S. C. Edman Tsang, Department of Chemistry, University of Oxford, Oxford, OX1 3QR (UK)

      K. K. L. Yung, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong (China)

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  • This work was supported by the Research Grant Council of Hong Kong (HKBU222610 to K.K.L.Y., HKBU201309 to H.W.L.) and Mini Area of Excellent Scheme (RC/AOE/08-09/02 to K.K.L.Y.).

Abstract

original image

It stems from the magnetism: The extraction of stem/progenitor cells from the brain of live animals is possible using antibodies conjugated to magnetic nanoparticles (Ab-MNPs). The Ab-MNPs are introduced to a rat's brain with a superfine micro-syringe. The stem cells attach to the Ab-MNPs and are magnetically isolated and removed. They can develop into neurospheres and differentiate into different types of cells outside the subject body. The rat remains alive and healthy.

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