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Rapid High-Specificity microRNA Detection Using a Two-stage Isotachophoresis Assay

Authors

  • Giancarlo Garcia-Schwarz,

    1. Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)
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  • Prof. Juan G. Santiago

    Corresponding author
    1. Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)
    • Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)

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  • We gratefully acknowledge support from the National Science Foundation under contract number CBET-1159092. G.G.-S. is supported by a Shustek Stanford Graduate Fellowship.

Abstract

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Focusing in on the small: A two-stage microRNA detection assay uses electrokinetic focusing to speed up hybridization and a functionalized hydrogel for affinity purification. This method detects microRNAs in 15 minutes with single-nucleotide specificity, and processes only 5 ng of total RNA.

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