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Contact-Mediated Quenching for RNA Imaging in Bacteria with a Fluorophore-Binding Aptamer

Authors


  • M.S. thanks the Alexander von Humboldt Foundation for a postdoctoral fellowship.

Abstract

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Bind and shine: Sulforhodamine B was converted into a very efficient fluorescence turn-on probe exclusively by contact quenching. The fluorescence of the probe increases more than 100-fold upon binding to a sulforhodamine-binding RNA aptamer (SRB-2). The probe and the SRB-2 aptamer were used to monitor transcriptions in real-time and to image RNA in live bacteria.

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