The authors acknowledge the support of the BBSRC Rasor Grant (BB/C511572/1) and the University of Glasgow Kelvin/Smith Scholarship Scheme and Wellcome Trust as part of their grant to the Wellcome Trust Centre for Molecular Parasitology (085349). Dr. Neale acknowledges the support of a RAEng research fellowship. The authors thank Dr. Lisa C. Ranford-Cartwright and Elizabeth Peat (Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow) for the access to blood samples.
Shape-Dependent Optoelectronic Cell Lysis†
Article first published online: 8 JAN 2014
© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Angewandte Chemie International Edition
Volume 53, Issue 3, pages 842–846, January 13, 2014
How to Cite
Kremer, C., Witte, C., Neale, S. L., Reboud, J., Barrett, M. P. and Cooper, J. M. (2014), Shape-Dependent Optoelectronic Cell Lysis. Angew. Chem. Int. Ed., 53: 842–846. doi: 10.1002/anie.201307751
- Issue published online: 8 JAN 2014
- Article first published online: 8 JAN 2014
- Manuscript Received: 3 SEP 2013
- Funded Access
- BBSRC. Grant Number: BB/C511572/1
- Wellcome Trust. Grant Number: 085349
- cell enrichment;
- electrical cell lysis;
We show an electrical method to break open living cells amongst a population of different cell types, where cell selection is based upon their shape. We implement the technique on an optoelectronic platform, where light, focused onto a semiconductor surface from a video projector creates a reconfigurable pattern of electrodes. One can choose the area of cells to be lysed in real-time, from single cells to large areas, simply by redrawing the projected pattern. We show that the method, based on the “electrical shadow” that the cell casts, allows the detection of rare cell types in blood (including sleeping sickness parasites), and has the potential to enable single cell studies for advanced molecular diagnostics, as well as wider applications in analytical chemistry.