These authors contributed equally to this work.
Cascade Reactions in Multicompartmentalized Polymersomes†
Article first published online: 19 NOV 2013
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 53, Issue 1, pages 146–150, January 3, 2014
How to Cite
Peters, R. J. R. W., Marguet, M., Marais, S., Fraaije, M. W., van Hest, J. C. M. and Lecommandoux, S. (2014), Cascade Reactions in Multicompartmentalized Polymersomes. Angew. Chem. Int. Ed., 53: 146–150. doi: 10.1002/anie.201308141
R.J.R.W.P. thanks the Dutch Science Foundation (NWO) for funding. M.M. and S.L. thank the French Ministère de l’Enseignement Supérieur et de la Recherche and the Precision Polymer Materials P2M program from the European Science Foundation for funding. J.C.M.v.H. acknowledges funding from the Ministry of Education, Culture and Science (Gravity program 024.001.035). M. van Eldijk is thanked for help with size-exclusion chromatography measurements.
- Issue published online: 23 DEC 2013
- Article first published online: 19 NOV 2013
- Manuscript Received: 16 SEP 2013
- Dutch Science Foundation (NWO)
- French Ministère de l’Enseignement Supérieur et de la Recherche
- European Science Foundation
- Ministry of Education, Culture and Science. Grant Number: 024.001.035
- artificial cells;
- cascade reactions;
- enzyme catalysis;
Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.