Get access

Cascade Reactions in Multicompartmentalized Polymersomes

Authors

  • Ruud J. R. W. Peters,

    1. Radboud University Nijmegen, Institute for Molecules and Materials, Bio-organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Maïté Marguet,

    1. Université de Bordeaux, LCPO, UMR 5629, 33600 Pessac (France)
    2. CNRS, LCPO, UMR 5629, 33600 Pessac (France)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Sébastien Marais,

    1. Bordeaux Imaging Center Université Bordeaux Segalen, CNRS UMS3420—Inserm US004, Institut François Magendie, 146 rue Léo-Saignat, 33077 Bordeaux Cedex (France)
    Search for more papers by this author
  • Prof. Marco W. Fraaije,

    1. University of Groningen, Biomolecular Sciences and Biotechnology Institute, Laboratory of Biochemistry, Nijenborgh 4, 9747 AG Groningen (The Netherlands)
    Search for more papers by this author
  • Prof. Jan C. M. van Hest,

    Corresponding author
    1. Radboud University Nijmegen, Institute for Molecules and Materials, Bio-organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands)
    • Jan C. M. van Hest, Radboud University Nijmegen, Institute for Molecules and Materials, Bio-organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands)

      Sébastien Lecommandoux, Université de Bordeaux, LCPO, UMR 5629, 33600 Pessac (France)

    Search for more papers by this author
  • Prof. Sébastien Lecommandoux

    Corresponding author
    1. Université de Bordeaux, LCPO, UMR 5629, 33600 Pessac (France)
    2. CNRS, LCPO, UMR 5629, 33600 Pessac (France)
    • Jan C. M. van Hest, Radboud University Nijmegen, Institute for Molecules and Materials, Bio-organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands)

      Sébastien Lecommandoux, Université de Bordeaux, LCPO, UMR 5629, 33600 Pessac (France)

    Search for more papers by this author

  • R.J.R.W.P. thanks the Dutch Science Foundation (NWO) for funding. M.M. and S.L. thank the French Ministère de l’Enseignement Supérieur et de la Recherche and the Precision Polymer Materials P2M program from the European Science Foundation for funding. J.C.M.v.H. acknowledges funding from the Ministry of Education, Culture and Science (Gravity program 024.001.035). M. van Eldijk is thanked for help with size-exclusion chromatography measurements.

Abstract

Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.

Get access to the full text of this article

Ancillary