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Cover Picture: LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes (Angew. Chem. Int. Ed. 43/2013)



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Large asymmetric assemblies are difficult to prepare recombinantly and result in complicated NMR spectra that cannot be analyzed in detail. Both issues are addressed simultaneously in the LEGO-NMR method described by R. Sprangers and co-workers in their Communication on page 11401 ff. The method is based on sequential co-expression of NMR active and NMR invisible protein subunits of a large assembly and significantly simplifies both amide backbone and methyl side-chain spectra.