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Rapid Generation of Cell Gradients by Utilizing Solely Nanotopographic Interactions on a Bio-Inert Glass Surface

Authors

  • Dr. Gao Yang,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (P.R. China)
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  • MSc. Yanhua Cao,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
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  • Dr. Junbing Fan,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
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  • Dr. Hongliang Liu,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
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  • Dr. Feilong Zhang,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (P.R. China)
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  • Dr. Pengchao Zhang,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    2. University of Chinese Academy of Sciences, Beijing 100049 (P.R. China)
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  • Dr. Chao Huang,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
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  • Prof. Lei Jiang,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
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  • Prof. Shutao Wang

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    • Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)

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  • This research was supported by the National Research Fund for Fundamental Key Projects (2012CB933800, 2013CB933000, and 2012CB934100), the National Natural Science Foundation (21121001, 21127025, 21175140, and 20974113), the Key Research Program of the Chinese Academy of Sciences (KJZD-EW-M01), and the National High Technology Research and Development Program of China (863 Program) (2013AA031903).

Abstract

The control of cell gradients is critical for understanding many biological systems and realizing the unique functionality of biomimetic implants. Herein, we report a nanotopographic gradient strategy that can rapidly generate cell gradients on a nanodendritic silica substrate without any chemical modification. We can achieve controllable cell gradients within only half an hour of cell incubation solely induced by the topographic effect of the gradient nanodendrites. We also demonstrate that cell gradients can be modulated by the combination of nanotopographic and chemical gradients. The results reveal that the enhanced topographic interactions between the nanodentritic structure and nanoscaled filopodia of the cells mainly contribute to the generation of cell gradients.

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