These authors contributed equally to this work.
Construction of a Live-Attenuated HIV-1 Vaccine through Genetic Code Expansion†
Article first published online: 8 APR 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 53, Issue 19, pages 4867–4871, May 5, 2014
How to Cite
Wang, N., Li, Y., Niu, W., Sun, M., Cerny, R., Li, Q. and Guo, J. (2014), Construction of a Live-Attenuated HIV-1 Vaccine through Genetic Code Expansion. Angew. Chem. Int. Ed., 53: 4867–4871. doi: 10.1002/anie.201402092
This work was supported by a grant from the Nebraska Research Initiative and a New Faculty Startup Fund to J.G. from the Chemistry Department of the University of Nebraska–Lincoln.
- Issue published online: 2 MAY 2014
- Article first published online: 8 APR 2014
- Manuscript Received: 4 FEB 2014
- attenuated vaccine;
- genetic code expansion;
- HIV-1 vaccine;
- unnatural amino acid;
- virus engineering
A safe and effective vaccine against human immunodeficiency virus type 1 (HIV-1) is urgently needed to combat the worldwide AIDS pandemic, but still remains elusive. The fact that uncontrolled replication of an attenuated vaccine can lead to regaining of its virulence creates safety concerns precluding many vaccines from clinical application. We introduce a novel approach to control HIV-1 replication, which entails the manipulation of essential HIV-1 protein biosynthesis through unnatural amino acid (UAA*)-mediated suppression of genome-encoded blank codon. We successfully demonstrate that HIV-1 replication can be precisely turned on and off in vitro.