[NiFe]Hydrogenase from Citrobacter sp. S-77 Surpasses Platinum as an Electrode for H2 Oxidation Reaction

Authors

  • Dr. Takahiro Matsumoto,

    1. International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan) http://www.cstm.kyushu-u.ac.jp/ogo/
    2. Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan)
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  • Shigenobu Eguchi,

    1. Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan)
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  • Dr. Hidetaka Nakai,

    1. Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan)
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  • Prof. Takashi Hibino,

    1. Graduate School of Environmental Studies, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601 (Japan)
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  • Dr. Ki-Seok Yoon,

    1. International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan) http://www.cstm.kyushu-u.ac.jp/ogo/
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  • Prof. Seiji Ogo

    Corresponding author
    1. International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan) http://www.cstm.kyushu-u.ac.jp/ogo/
    2. Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan)
    3. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012 (Japan)
    • International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395 (Japan) http://www.cstm.kyushu-u.ac.jp/ogo/

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  • Citrobacter sp. S-77 takes its identifying initial from Saori Ogo, whose assistance was invaluable to the hunt for new hydrogenases. This work was supported by Grants-in-Aid: 26000008 (Specially promoted Research), 26810038, 26410074, 25620047, 25248017, and 24109016 from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) (Japan), the World Premier International Research Center Initiative (WPI), and the Basic Research Programs CREST Type from JST (Japan).

Abstract

Reported herein is an electrode for dihydrogen (H2) oxidation, and it is based on [NiFe]Hydrogenase from Citrobacter sp. S-77 ([NiFe]S77). It has a 637 times higher mass activity than Pt (calculated based on 1 mg of [NiFe]S77 or Pt) at 50 mV in a hydrogen half-cell. The [NiFe]S77 electrode is also stable in air and, unlike Pt, can be recovered 100 % after poisoning by carbon monoxide. Following characterization of the [NiFe]S77 electrode, a fuel cell comprising a [NiFe]S77 anode and Pt cathode was constructed and shown to have a a higher power density than that achievable by Pt.

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