Au@Pt Nanoparticle Encapsulated Target-Responsive Hydrogel with Volumetric Bar-Chart Chip Readout for Quantitative Point-of-Care Testing

Authors

  • Prof. Zhi Zhu,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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    • These authors contributed equally to this work.

  • Zhichao Guan,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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    • These authors contributed equally to this work.

  • Shasha Jia,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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    • These authors contributed equally to this work.

  • Zhichao Lei,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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  • Dr. Shuichao Lin,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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  • Huimin Zhang,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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  • Yanli Ma,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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  • Prof. Zhong-Qun Tian,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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  • Prof. Chaoyong James Yang

    Corresponding author
    1. State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
    • State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)

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  • This work was supported by the National Basic Research Program of China (2010CB732402, 2013CB933703), the National Science Foundation of China (91313302, 21205100, 21275122), and the National Science Foundation for Distinguished Young Scholars of China (21325522).

Abstract

Point-of-care testing (POCT) with the advantages of speed, simplicity, portability, and low cost is critical for the measurement of analytes in a variety of environments where access to laboratory infrastructure is lacking. While qualitative POCTs are widely available, quantitative POCTs present significant challenges. Here we describe a novel method that integrates an Au core/Pt shell nanoparticle (Au@PtNP) encapsulated target-responsive hydrogel with a volumetric bar-chart chip (V-Chip) for quantitative POCT. Upon target introduction, the hydrogel immediately dissolves and releases Au@PtNPs, which can efficiently catalyze the decomposition of H2O2 to generate a large volume of O2 to move of an ink bar in the V-Chip. The concentration of the target introduced can be visually quantified by reading the traveling distance of the ink bar. This method has the potential to be used for portable and quantitative detection of a wide range of targets without any external instrument.

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