Amides as a New Type of Backbone Modification in Oligonucleotides (pages 226–229)
Dr. Alain De Mesmaeker, Dr. Adrian Waldner, Dr. Jacques Lebreton, Dr. Pascale Hoffmann, Dr. Valérie Fritsch, Dr. Romain M. Wolf and Dr. Susan M. Freier
Article first published online: 22 DEC 2003 | DOI: 10.1002/anie.199402261
No decrease in the affinity for the complementary RNA target is observed when the natural phosphodiester linkages in oligonucleotides are replaced with amide units. This was found in investigations of oligonucleotides containing one or more modified components 1. In addition these oligonucleotides are roughly ten times more resistant towards 3′-exonucleases.