Enantioselective Reduction of β-Keto Acids with Engineered Streptomyces coelicolor (pages 1121–1125)
Kevin I. Booker-Milburn, Rebecca Gillan, Meriel Kimberley, Takaaki Taguchi, Koji Ichinose, G. Richard Stephenson, Yutaka Ebizuka and David A. Hopwood
Article first published online: 11 JAN 2005 | DOI: 10.1002/anie.200462076
Compelling evidence for the intermediacy of the free β-keto acid 1, rather than the corresponding enzyme-bound thiolate as previously proposed, in the biosynthesis of the antibiotic actinorhodin (2) was obtained from studies of the enantioselective reduction of a range of β-keto acids by the engineered strain of S. coelicolor CH999/pIJ5675. This excellent whole-cell biotransformation system gave the desired S β-hydroxy acids with >95 % ee.