Measuring Biomolecular Binding Events with a Compact Disc Player Device (pages 270–273)
Sebastian A. Lange, Günter Roth, Silke Wittemann, Thilo Lacoste, Andreas Vetter, Jürgen Grässle, Susanne Kopta, Matthias Kolleck, Beate Breitinger, Manfred Wick, J. K. Heinrich Hörber, Stefan Dübel and André Bernard
Version of Record online: 21 DEC 2005 | DOI: 10.1002/anie.200501243
Read the fine print: Biomolecular binding events can be counted with a readout system based on compact disc (CD) pickup technology. An immunoassay of C-reactive protein was performed by microcontact printing of antibodies onto a support, autometallography, and imaging by a CD reader mounted on an optical microscope (see picture). The dynamic range was four orders of magnitude with a detection limit of 1 pM.