Polymer-Based Elemental Tags for Sensitive Bioassays (pages 6111–6114)
Xudong Lou, Guohua Zhang, Isaac Herrera, Robert Kinach, Olga Ornatsky, Vladimir Baranov, Mark Nitz and Mitchell A. Winnik
Version of Record online: 29 MAY 2007 | DOI: 10.1002/anie.200700796
It's elemental: A water-soluble polymer bearing multiple metal-chelating ligands has been used as a tag for bioassays with inductively coupled plasma mass spectrometry. The tag was covalently conjugated to antibodies, and the polymer–antibody constructs were loaded with lanthanide ions (Ln3+) and used for the simultaneous assay of five orthogonally labeled antibodies against cell surface antigens that differ in abundance by more than two orders of magnitude.