Photochemical Surface Patterning by the Thiol-Ene Reaction (pages 4421–4424)
Pascal Jonkheijm, Dirk Weinrich, Maja Köhn, Hans Engelkamp, Peter C. M. Christianen, Jürgen Kuhlmann, Jan C. Maan, Dirk Nüsse, Hendrik Schroeder, Ron Wacker, Rolf Breinbauer, Christof M. Niemeyer and Herbert Waldmann
Article first published online: 21 APR 2008 | DOI: 10.1002/anie.200800101
The photochemical coupling of olefin-capped (bio)molecules to surface-bound thiols can be used to control protein immobilization on length scales of centimeters to sub-micrometers (see the fluorescence microscopy image of a nanopattern after treatment with labeled streptavidin). Two enzymes immobilized on the resulting patterns retained their enzymatic activity and underwent protein–protein interactions similar to those in the solution phase.