Two-Photon Fluorescent Probes for Intracellular Free Zinc Ions in Living Tissue (pages 5167–5170)
Hwan Myung Kim, Mun Sik Seo, Myoung Jin An, Jin Hee Hong, Yu Shun Tian, Joon Ho Choi, Ohyun Kwon, Kyoung J. Lee and Bong Rae Cho
Article first published online: 4 JUN 2008 | DOI: 10.1002/anie.200800929
The living image: The efficient two-photon probes AZn1 and AZn2 (see picture for rat brain tissue) show 24- to 52-fold two-photon excited fluorescence enhancement in response to Zn2+. They can selectively detect intracellular free Zn2+ ions in live cells and in living tissues at a depth of 80–150 μm without interference from other metal ions and the membrane-bound probes.